Entomopathogenic fungi, culturing

April 2008. A request from Zambia for information on the culturing of entomopathogenic fungi. A Calidea has been found with a white mycelium growing from its spiracles.

There is information on PestNet Summaries on the culture of entomopathogenic fungi.

staging.pestnet.org/Summaries/Pests/PestsEntities/Fungi/CulturetechniquesPaecilomyces/tabid1279/Default

A recipe was provided:
Sucrose 20g
Agar 15g
Peptone 5g
MgSO₄ 0.25g
K²HPO₄ 0.25g
Water 1 liter.

First mixed all ingredients and autoclave the medium. EPF are successfully grown in this medium.